PCR Gradient Thermocycler (ABI)

Description

A PCR (Polymerase Chain Reaction) gradient thermocycler is a specialized instrument designed for amplifying DNA sequences. It allows for simultaneous testing of multiple annealing temperatures, optimizing PCR conditions for various samples.

Features of a Good PCR Gradient Thermocycler

1. Gradient Capability

   – Ability to set a temperature gradient across a block (e.g., 4°C to 100°C) to optimize annealing conditions.

2. Accuracy and Precision

   – High precision in temperature control, typically ±0.5°C to ensure reproducibility.

3. Multiple Sample Capacity

   – Configurations to accommodate various plate formats (e.g., 96-well, 384-well).

4. User-Friendly Interface

   – Intuitive touchscreen or software for easy programming and tracking.

5. Fast Heating and Cooling

   – Quick thermocycling to reduce overall run times, with ramp rates generally around 3°C to 5°C per second.

6. Data Logging

   – Options for storing protocols and reviewing temperature profiles post-experiment.

7.Flexible Programming Options

   – Supports various PCR protocols, including steps for denaturation, annealing, and extension.

 Brand: Applied Biosystems – Veriti™ 96-Well Thermal Cycler

 Technical Specifications

– Temperature Range: 4°C to 100°C

– Temperature Gradient Range: 30°C across the block

– Block Formats: 96-well, compatible with various PCR plates and tubes

– Heating Rate: 3°C to 5°C per second

– Cooling Rate: Adjustable settings for rapid cooling

– User Interface: 7-inch color touchscreen with intuitive programming

– Data Storage: Up to 1000 protocols

– Weight: Approximately 15 kg

– Power Supply: 100-240 V AC, suitable for global use

PCR gradient thermocyclers are vital in molecular biology labs for optimizing PCR conditions, ensuring successful DNA amplification for downstream applications.