NanoDrop Spectrophotometer (Thermo Fisher)

Description

The NanoDrop spectrophotometer is a compact instrument designed for measuring the concentration and purity of nucleic acids and proteins in small sample volumes (typically 1-2 µL). It provides precise absorbance measurements at specific wavelengths, allowing for quick assessments without requiring cuvettes.

Key Features

1. Small Sample Volume

   – Requires only 1-2 µL for analysis, minimizing sample waste.

2. Fast Measurements

   – Results are available in seconds, providing rapid analysis for high-throughput workflows.

3. Broad Wavelength Range

   – Measures absorbance from 230 nm to 850 nm, accommodating various applications.

4. Automatic Computing

   – Automatically calculates concentrations and purity ratios (A260/A280, A260/A230).

5. User-Friendly Software

   – Intuitive software interface for data entry and results management.

6. Built-in Method Protocols

   – Pre-programmed methods for DNA, RNA, and protein quantification ensure ease of use.

7. Data Export Options

   – Results can be saved or exported in multiple formats for further analysis.

 Example Model: Thermo Fisher Scientific NanoDrop 2000/2000C

 Technical Specifications

– Wavelength Range: 200 nm to 850 nm

– Sample Volume: 1-2 µL

– Dynamic Range: 2 ng/µL to 15,000 ng/µL for nucleic acids

– Accuracy: Typically ±1% for nucleic acids

– Repeatability: CV < 1% for multiple measurements

– Dimensions: Compact design, approximately 30 x 20 x 15 cm

– Weight: Around 3 kg

– Power Requirement: 100-240 V AC, 50/60 Hz

The NanoDrop spectrophotometer is essential for labs engaged in molecular biology, helping researchers efficiently quantify nucleic acids and proteins with high accuracy.